Figure 1From: A base-calling algorithm for Tm-shifted melting curve SNP assayTypical melting curve plots of three alleles. (A) allele 1; (B) allele 2; (C) allele 3. The horizontal axis represents the temperature (T). The vertical axis is the fluorescent intensity derivative (M) w.r.t. temperature. The major peaks of the curve occur in the proximity of theoretical melting temperatures of the two allele-specific PCR duplex.Back to article page