Table 2 Description of primers and probes for validated candidate reference genes
Gene name | Sequence of primers and probes* | Amplicon size (bp) | E |
|---|---|---|---|
HCFC1 | f-CCACATCGACTACACCACCAA | 212 | 1.90 |
| Â | r-CAGCTTCCTCTCACTGACCATC | Â | Â |
| Â | pr-(FAM)-CGCCATCA(T-BHQ1)CTTCCGCATCGCC-(P) | Â | Â |
TPT1 | f-ATCAGCCACGATGAGATGTTC | 132 | 1.76 |
| Â | r-ATTTCCACCAATGAGCGAGTC | Â | Â |
| Â | pr-(FAM)-CGGACGGG(T-BHQ1)TGTGCCTGGAGGT-(P) | Â | Â |
RPL37A | f-AAGTCGGGATCGTCGGTAAA | 112 | 1.97 |
| Â | r-TTGCCACAGAAAGAGCAAGT | Â | Â |
| Â | pr-(FAM)-TTGAAA(T-BHQ1)CAGCCAGCACGCCAAGT-(P) | Â | Â |
PTMA | f-ACCACCCAACCCAAACCA | 238 | 1.85 |
| Â | r-TGGTCACACCACAAGTAAAGT | Â | Â |
| Â | pr-(FAM)-TCGGATGACCAAACCAGCC(T-BHQ1)TCGG-(P) | Â | Â |
RPL23A | f-CTGGAAGAGGCTGTGTATGAA | 120 | 1.96 |
| Â | r-TAGTAGATGGGTGTGTGAGGAC | Â | Â |
| Â | pr-(FAM)-AGGGGAG(T-BHQ1)GTGGATTGGCTGGC-(P) | Â | Â |
TBP | f-TTCGGAGAGTTCTGGGATTGTA | 227 | 1.83 |
| Â | r-TGGACTGTTCTTCACTCTTGGC | Â | Â |
| Â | pr-(FAM)-CCGTGGTTCG(T-BHQ1)GGCTCTCTTATCCTCAT-(P) | Â | Â |
GUSB | f-CTCATTTGGAATTTTGCCGATT | 81 | 1.87 |
| Â | r-CCGAGTGAAGATCCCCTTTTTA | Â | Â |
| Â | pr-(FAM)-TGAACAG(T-BHQ1)CACCGACGAGAGTGCTGG-(P) | Â | Â |
SF3A1 | f-AAGGGTCCAGTGTCCATCAAAGT | 224 | 1.88 |
| Â | r-GCCATGTTGTAGTAAGCCAGTGAG | Â | Â |
| Â | pr-(FAM)-ACCAGGTC(T-BHQ1)CTGTCATTAAGGTGAAG-(P) | Â | Â |
MRPL19 | f-TGTTTGCGGTTGTTAGTTCAC | 239 | 1.87 |
| Â | r-ACATTTCTGCTTGCCCTTCC | Â | Â |
| Â | pr-(FAM)-CATTGCC(T-BHQ1)ACTGCTTACGATGAGTGC-(P) | Â | Â |
ACTB | f-CTGGAACGGTGAAGGTGACA | 140 | 1.87 |
| Â | r-AAGGGACTTCCTGTAACAACGCA | Â | Â |
| Â | pr-(FAM)-TGGAGCGAGCA(T-BHQ1)CCCCCAAAGT-(P) | Â | Â |
GAPDH | f-GAAGGTGAAGGTCGGAGTC | 226 | 1.99 |
r-GAAGATGGTGATGGGATTTC | |||
| Â | pr-(FAM)-CAAGCTTCCCG(T- BHQ1)TCTCAGCC-(P) | Â | Â |
RPLP0 | f-CTGATCCATCTGCCTTTGTG | 116 | 1.89 |
r-GTCCGACTCCTCCGACTCTT | |||
| Â | pr-(FAM)-AGCCCCAGC(T-BHQ1)AAGGTTGAAGCCA-(P) | Â | Â |
TFRC | f-AAAGGAAATGGGCCTGAGTTTA | 92 | 1.96 |
r-CATTCCCGAAATCTGTTGTTAG | |||
| Â | pr-(FAM)-TGGCTGTATTC(T-BHQ1)GCTCGTGGAGA-(P) | Â | Â |
PSMC4 | f-AGCTCTACAAGCAGATCG | 80 | 1.95 |
r-CAACATGGTCTTCCCACA | |||
| Â | pr-(FAM)-AGGCGTCCTCATG(T-BHQ1)ATGGCCCACCT-(P) | Â | Â |
HUWE1 | f- CAGAGTTGGACAGAGTGAAA | 137 | 2.10 |
r- TACACAGAGAGAGGAGGACA | |||
| Â | pr-(FAM)-TCGTTCCATCTCCG(T-BHQ1)AAACCAG-(P) | Â | Â |
EEF1A1 | f-TGAAAACTACCCCTAAAAGCCA | 208 | 1.91 |
r- TATCCAAGACCCAGGCATACT | |||
| Â | pr- (FAM)-TAGATTCGGGCAAG(T-BHQ1)CCACCA-(P) | Â | Â |
RPS23 | f- CGGTGCTTCTCTCTTTCGCT | 110 | 2.00 |
r- ATGCCACTTCTGGTCTCGT | |||
| Â | pr- (FAM)-AGTGTCG(T-BHQ1)GGACTTCGTACTGC-(P) | Â | Â |
RPL39 | f- TTATGCTGTCTGAAGGTCACGA | 120 | 1.85 |
r- AATCCAGCCAACCAACGTG | |||
| Â | pr- (FAM)-TGGAGATT(T-BHQ1)CGACGTGTTTTCCTCTC-(P) | Â | Â |