Figure 3
From: Quantitative analysis of histone exchange for transcriptionally active chromatin
Histone exchange occurs more readily in chromatin containing transcription activating PTMs. (A) The average and standard error of isotopically light arginine containing peptides for bulk H3, H3K9acK14ac, H2B-TAP, and 15 non-specifically associating proteins are plotted as a function of formaldehyde cross-linking. (B) Plot of bulk H4, H4K12acK16ac, H2B-TAP, and 15 non-specific proteins as a function of formaldehyde cross-linking. Levels approaching 100% light peptides indicate minimal histone exchange while levels at ~50% light peptides reflect rapid exchange.