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Table 3 List of identified proteins with their accession numbers, theoretical and experimental molecular weights, pIs,

From: Proteome analysis of bronchoalveolar lavage in pulmonary langerhans cell histiocytosis

No. of spot Protein name AC Theoretical
pI/Mr (KDa)
Experimental
pI/Mr (KDa)
Mascot search result Mean %V ± SD × 10-4 1-way ANOVA p value Folds in Localization
      No. of matched peptide Sequence coverage (%) Score nsc sc PLCH   Nsc-sc Nsc-PLCH Sc-PLCH  
PLCH > nsc and/or sc                
1 Kininogen-1, fragment N-term P01042 6.34 4.95 11 18 118 543±105§ 225±214¥ 5393±1864¥§ 8.97E-06 2.41 9.93§ 23.96¥ Secreted-extracellular space
    72996 61092            
2 Ig alpha-1 chain C region P01876 6.08 5.98 8 25 116 879±788§ 206±207¥ 3179±1685¥§ 0,002 4.26 3.61§ 15.43¥ Secreted
    38486 60221            
3 Pigment epithelium P36955 5.97 5.61 8 24 111 405±176§ 281±87¥ 1230±693¥§ 0,007 1.44 3.03§ 4.37¥ Secreted
    46484 49075            
4 Pigment epithelium P36955 5.97 5.69 8 21 114 326±211§ 307±182¥ 767±260¥§ 0,009 1.06 2.35§ 2.49¥ Secreted
    46484 49222            
5 Haptoglobin, fragment c-term. P00738 6.13 5.26 12 31 114 446±69§ 432±180¥ 2062±1186¥§ 0,003 1.03 4.62§ 4.77¥ Plasma
    45861 40355            
6 Creatine kinase B-type P12277 5.34 5.31 7 26 108 267±146§ 161±93¥ 749±306¥§ 0,001 1.65 2.80§ 4.65¥ Cytoplasm
    42902 41841            
7 Annexin A3 P12429 5.63 5.59 8 32 111 158±159§ 127±54¥ 530±318¥§ 0,017 1.24 3.35§ 4.17¥ Cytoplasm
    36524 31681            
8 Alpha-1 anti tripsin P01009 5.37 4.84 15 46 212 1491±1178§ 299±413¥ 5425±817¥§ 1,79E-06 4.98 3.63§ 18.14¥ Plasma
    46878 53626            
9 Alpha-1 anti tripsin P01009 5.37 4.8 8 27 107 495 ± 979§ 28 ± 64¥ 2644 ± 1473¥§ 0,003 17.6 5.34§ 94.42¥ Plasma
    46878 54359            
10 Ceruloplasmin P00450 5.44 5.1 11 13 134 135±118§ 154±149¥ 496±121¥§ 0,001 1.14 3.67§ 3.22¥ Plasma
    122983 131973            
11 Alpha-1-antitrypsin P01009 5.37 4.96 13 41 175 288±221§ 423±204¥ 1359±551¥§ 0,001 1.46 4.71§ 3.21¥ Plasma
    46878 58032            
12 Serotransferrin P02787 6.81 6.05 8 11 102 415±322§ 162±219¥ 2655±1223¥§ 0,0003 2.56 6.39§ 16.38¥ Plasma
    79280 60048            
13 Serotransferrin P02787 6.81 6 11 19 112 623±170§ 672±576¥ 3255±2316¥§ 0,016 1.07 5.22§ 4.84¥ Plasma
    79280 80091            
14 Pyruvate kinase isozymes P14618 7.96 6.47 11 28 105 276±236§ 642±290¥ 1391±596¥§ 0,003 2.32 5.03§ 2.16¥ Cytoplasm-Nucleus
    58470 58174            
15 Apolipoprotein A-I P02647 5.56 5.03 8 26 99 179±103§ 276±197¥ 924±371¥§ 0,0009 1.54 5.16§ 3.34¥ Plasma
    30759 23065            
16 Hemoglobin subunit beta P68871 6.75 6.77 11 77 155 109 ± 131§ 328 ± 123¥ 650 ± 233¥§ 0,001 3 5.96§ 1.98¥ Blood
    16102 11120            
17 Ceruloplasmin P00450 5.44 5.13 15 18 123 259±183§ 180±179¥ 564±99¥§ 0,005 1.43 2.17§ 3.13¥ Plasma
    122983 130654            
18 Alpha-1-antitrypsin P01009 5.37 4.7 11 32 143 277±290 93±77¥ 599±288¥ 0,018 2.97 2.16 6.44¥ Plasma
    46878 50566            
19 Actin, cytoplasmic 1 P60709 5.29 5.07 9 28 107 867±437 320±272¥ 1071±348¥ 0,016 2.7 1.23 3.34¥ Cytoskeleton
    42052 40664            
20 Ceruloplasmin P00450 5.44 5.16 10 11 101 306±223 201±151¥ 537±137¥ 0,02 1.52 1.75 2.67¥ Plasma
    122983 130000            
21 Serotransferrin P02787 6.81 6.07 16 22 166 1085±228§ 1831±559 4471±2939§ 0,022 1.68 4.12§ 2.44 Plasma
    79280 80.091            
22 Serotransferrin P02787 6.81 6.14 18 23 188 2376±300§ 3235±879 4969±1743§ 0,01 1.36 2.09§ 1.53 Plasma
    79280 79397            
23 Fibrinogen gamma chain P02679 5.37 5.52 10 28 133 511±354§ 766±671 2649±2055§ 0,039 1.49 5.18§ 3.45 Plasma
    52106 53481            
24 Alpha-1-antitrypsin P01009 5.37 5.29 8 27 121 197±222§ 641±651 1938±1200§ 0,01 3.25 9.83§ 3.02¥ Plasma
    46878 54009            
  1. Mascot search results, mean and standard deviations, statistical p values and number of folds of protein expression in the three groups. Statistical significant differences between smoking controls (sc) and no-smoking controls (nsc) are visualized by *, statistical differences between smoking controls and Pulmonary Langerhans cell Histiocytosis (PLCH) are visualized by ¥and significant differences occurring between no-smoking controls and Pulmonary Langerhans cell Histiocytosis are shown by §. The proteins quantitatively more abundant in PLCH than in non-smoker and/or smoker control samples are reported in table 3.