Figure 2

Comparison of WGS, 16S-WGS and 16S-HTS datasets to study bacterial community of the saliva sample. Group-average clustering of data was performed using a Bray-Curtis similarity matrix in PRIMER-E (Plymouth), based on square-root-transformed genera abundance. Only genera which occurred at a frequency of > 0.1% in at least one of 14 subsets were included in the analysis. V1 and V3 designate the sequenced hypervariable region of 16S rDNA. 20, 25 and 30 indicate the number of PCR cycles performed. F, forward reads; R, reverse reads.