|
Datasets to which the F and R 30-cycle V3 subsets were compared
|
Average similaritya ± SD (%)
|
Pearson's correlation coefficient (F, R)b
|
|---|
|
Itself (F vs R)
|
94.8
|
0.998
|
|
25-cycle V3
|
97.1 ± 0.3
|
0.998, 0.999
|
|
20-cycle V3
|
93.2 ± 1.5
|
0.974, 0.977
|
|
30-cycle V1
|
82.7 ± 0.8
|
0.987, 0.990
|
|
16S-WGS
|
68.5 ± 3.7
|
0.933, 0.958
|
|
WGS
|
64.1 ± 0.5
|
0.650, 0.652
|
|
V3 from other individualsc
|
64.8 ± 3.5
|
0.453-0.871, 0.458-0.867
|
- F, forward run; R, reverse run.
- a The genera frequency were square-root transformed and used to construct the Bray-Curtis similarity matrix. When applicable, the similarities were calculated separately for the pairs of forward and the pairs of reversed subsets prior to averaging.
- b Based on untransformed data.
- c Samples 1.1, 2.1, 3.1, 4.1 and 5.1 from a previous study [21] in which the initial PCR cycles were carried out in the same conditions as those in the present work. Then, 28 cycles were performed using bar-coded primers and the amplicons were sequenced by the Roche/454 platform. The average similarity observed among these five microbiomes was 65.2 ± 7.2%.
