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Table 1 RNA concentration, RNA integrity number and 3′/5′ ratios for fresh-frozen and formalin-fixed, paraffin-embedded tissue samples

From: An optimized workflow for improved gene expression profiling for formalin-fixed, paraffin-embedded tumor samples

Sample FF samples FFPET samples
  RNA conc. (ng/μL) RIN* 3′/5′ ratio RNA conc. (ng/μL) RIN 3′/5′ ratio
1 153 7.8 1.0 258 2.1 4.2
2 27 9.0 1.1 71 2.2 6.8
3 40 6.2 1.4 156 2.5 24.2
4 98 7.0 1.2 87 1.8 7.4
5 49 8.6 1.0 127 NA 5.1
6 73 7.3 1.0 96 NA 90.5
7 326 8.6 1.0 467 NA 90.8
8 87 6.8 1.2 168 6.2 44.3
9 34 5.4 1.5 74 NA 151.3
10 55 8.3 1.0 100 NA 90.8
11 23 6.9 1.2 53 2.6 9.0
12 120 7.9 1.0 162 2.8 72.2
13 58 7.7 1.1 71 2.6 17.1
14 110 6.9 1.1 178 2.5 11.3
15 121 7.8 1.1 219 2.3 16.6
16 175 7.1 1.2 349 2.3 16.1
  1. FF, fresh-frozen; FFPET, formalin-fixed, paraffin-embedded tissue; NA, not assessable; RIN, RNA integrity number.
  2. * RNA integrity is gauged using the RIN, which indicates the intactness of the sample by assigning a grade from 1 to 10, 10 being the most intact.