Journal of Clinical Bioinformatics

Table 1 RNA concentration, RNA integrity number and 3′/5′ ratios for fresh-frozen and formalin-fixed, paraffin-embedded tissue samples

From: An optimized workflow for improved gene expression profiling for formalin-fixed, paraffin-embedded tumor samples

Sample	FF samples			FFPET samples
	RNA conc. (ng/μL)	RIN*	3′/5′ ratio	RNA conc. (ng/μL)	RIN	3′/5′ ratio
1	153	7.8	1.0	258	2.1	4.2
2	27	9.0	1.1	71	2.2	6.8
3	40	6.2	1.4	156	2.5	24.2
4	98	7.0	1.2	87	1.8	7.4
5	49	8.6	1.0	127	NA	5.1
6	73	7.3	1.0	96	NA	90.5
7	326	8.6	1.0	467	NA	90.8
8	87	6.8	1.2	168	6.2	44.3
9	34	5.4	1.5	74	NA	151.3
10	55	8.3	1.0	100	NA	90.8
11	23	6.9	1.2	53	2.6	9.0
12	120	7.9	1.0	162	2.8	72.2
13	58	7.7	1.1	71	2.6	17.1
14	110	6.9	1.1	178	2.5	11.3
15	121	7.8	1.1	219	2.3	16.6
16	175	7.1	1.2	349	2.3	16.1

FF, fresh-frozen; FFPET, formalin-fixed, paraffin-embedded tissue; NA, not assessable; RIN, RNA integrity number.
* RNA integrity is gauged using the RIN, which indicates the intactness of the sample by assigning a grade from 1 to 10, 10 being the most intact.

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