Skip to main content

Table 4 Stability characteristics evaluated for the system of 18 individual genes and 2 “quasi-genes” – one corresponding to the selected system of reference genes, and the other corresponding to the system of reference genes used in OcotypeDX test

From: High-throughput identification of reference genes for research and clinical RT-qPCR analysis of breast cancer samples

 

Aggregate set of samples

 

ΔCt

geNorm

Haller

Norm Finder

Best gene*

ACTB

ACTB

PSMC4

ACTB

0.864

0.789

1.853

0.252

Selected subset

0.767

0.706

3.138

0.114

OncotypeDX subset

0.866

0.805

3.157

0.263

  1. *Best gene corresponds to gene with the least variability characteristic among 18 genes.