Table 3 Thermal conditions for TAIL-PCR
From: Development of detection method for novel fusion gene using GeneChip exon array
Pre-amplification | Primary TAIL-PCR | Secondary TAIL-PCR | ||||||
|---|---|---|---|---|---|---|---|---|
Step | Temperature (°C) | Time (min:sec) | Step | Temperature (°C) | Time (min:sec) | Step | Temperature (°C) | Time (min:sec) |
1 | 93 | 2:00 | 1 | 94 | 0:20 | 1 | 94 | 0:20 |
2 | 95 | 1:00 | 2 | 65 | 1:00 | 2 | 68 | 1:00 |
3 | 94 | 0:30 | 3 | 72 | 3:00 | 3 | 72 | 3:00 |
4 | 25 | 2:00 | 4 | To step 1 | 1 time | 4 | 94 | 0:20 |
5 | Ramping to 72 | 0.5°C/s | 5 | 94 | 0:20 | 5 | 68 | 1:00 |
6 | 72 | 3:00 | 6 | 68 | 1:00 | 6 | 72 | 3:00 |
7 | 94 | 0:30 | 7 | 72 | 3:00 | 7 | 94 | 0:20 |
8 | 60 | 1:00 | 8 | 94 | 0:20 | 8 | 50 | 1:00 |
9 | 72 | 3:00 | 9 | 68 | 1:00 | 9 | 72 | 3:00 |
10 | Go to step7 | 10 times | 10 | 72 | 3:00 | 10 | To step 1 | 7 times |
11 | 94 | 0:30 | 11 | 94 | 0:20 | 11 | 72 | 5:00 |
12 | 25 | 2:00 | 12 | 50 | 1:00 | Â | Â | Â |
13 | Ramping to 72 | 0.5°C/s | 13 | 72 | 3:00 |  |  |  |
14 | 72 | 3:00 | 14 | To step 5 | 13 times | Â | Â | Â |
15 | 94 | 0:20 | 15 | 72 | 5:00 | Â | Â | Â |
16 | 58 | 1:00 | Â | Â | Â | Â | Â | Â |
17 | 72 | 3:00 | Â | Â | Â | Â | Â | Â |
18 | Go to step 15 | 25 times | Â | Â | Â | Â | Â | Â |
19 | 72 | 5:00 | Â | Â | Â | Â | Â | Â |