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Table 3 Thermal conditions for TAIL-PCR

From: Development of detection method for novel fusion gene using GeneChip exon array

Pre-amplification Primary TAIL-PCR Secondary TAIL-PCR
Step Temperature (°C) Time (min:sec) Step Temperature (°C) Time (min:sec) Step Temperature (°C) Time (min:sec)
1 93 2:00 1 94 0:20 1 94 0:20
2 95 1:00 2 65 1:00 2 68 1:00
3 94 0:30 3 72 3:00 3 72 3:00
4 25 2:00 4 To step 1 1 time 4 94 0:20
5 Ramping to 72 0.5°C/s 5 94 0:20 5 68 1:00
6 72 3:00 6 68 1:00 6 72 3:00
7 94 0:30 7 72 3:00 7 94 0:20
8 60 1:00 8 94 0:20 8 50 1:00
9 72 3:00 9 68 1:00 9 72 3:00
10 Go to step7 10 times 10 72 3:00 10 To step 1 7 times
11 94 0:30 11 94 0:20 11 72 5:00
12 25 2:00 12 50 1:00    
13 Ramping to 72 0.5°C/s 13 72 3:00    
14 72 3:00 14 To step 5 13 times    
15 94 0:20 15 72 5:00    
16 58 1:00       
17 72 3:00       
18 Go to step 15 25 times       
19 72 5:00