Table 4 Primers for RT-PCR
From: Development of detection method for novel fusion gene using GeneChip exon array
| Target fusion gene | Primer name | Orientation | Sequence (5′- 3′) | Amplicon size |
|---|---|---|---|---|
| DOCK5-CDCA2 | DOCK5-exonl | Forward | GAGGAGCTGTAGCAGCCTTAGTCG | 371 bp |
| CDCA2-TAIL2 | Reverse | CTTGATGCATATGCAAATCTGGGTCATGACGC | ||
| DOCK5-CDCA2 | DOCK5-exon1 | Forward | GAGGAGCTGTAGCAGCCTTAGTCG | 760 bp |
| CDCA2-TAIL0 | Reverse | GCATTGCAGTTTTCCTTCTGCAGCTCC | ||
| ZMYND8-CEP250 | ZMYND8-exon18 | Forward | TACATCAGGAGGCMAGCGACA | 513 bp |
| CEP250-TAIL2 | Reverse | GCAGTGTCTCCAGGAGGGATACTCTC | ||
| ZMYND8-CEP250 | ZMYND8-exonl5 | Forward | GCCGCTTTTACCGAAGGAGACT | 1476 bp |
| CE P250-exon27 | Reverse | GCTGCTGCTCCGTGATATGAGA | ||
| RLF-ZMPSTE24 | RLF-TAIL2 | Forward | CCCCCAGGCTACTGCTTTATCAAAACTA | 445 bp |
| ZMPSTE24-exon3 | Reverse | CATAACCACAGAACCGTCCAGAAAG | ||
| RLF-ZMPSTE24 | RLF-exon1 | Forward | GTTGCCTACGCGCTGGTG | 2167 bp |
| ZMPSTE24-exon10 | Reverse | GATGTCCAGGATCTGTGACTGA |