Table 4 Primers for RT-PCR
From: Development of detection method for novel fusion gene using GeneChip exon array
Target fusion gene | Primer name | Orientation | Sequence (5′- 3′) | Amplicon size |
|---|---|---|---|---|
DOCK5-CDCA2 | DOCK5-exonl | Forward | GAGGAGCTGTAGCAGCCTTAGTCG | 371Â bp |
| Â | CDCA2-TAIL2 | Reverse | CTTGATGCATATGCAAATCTGGGTCATGACGC | Â |
DOCK5-CDCA2 | DOCK5-exon1 | Forward | GAGGAGCTGTAGCAGCCTTAGTCG | 760Â bp |
| Â | CDCA2-TAIL0 | Reverse | GCATTGCAGTTTTCCTTCTGCAGCTCC | Â |
ZMYND8-CEP250 | ZMYND8-exon18 | Forward | TACATCAGGAGGCMAGCGACA | 513Â bp |
| Â | CEP250-TAIL2 | Reverse | GCAGTGTCTCCAGGAGGGATACTCTC | Â |
ZMYND8-CEP250 | ZMYND8-exonl5 | Forward | GCCGCTTTTACCGAAGGAGACT | 1476Â bp |
| Â | CE P250-exon27 | Reverse | GCTGCTGCTCCGTGATATGAGA | Â |
RLF-ZMPSTE24 | RLF-TAIL2 | Forward | CCCCCAGGCTACTGCTTTATCAAAACTA | 445Â bp |
| Â | ZMPSTE24-exon3 | Reverse | CATAACCACAGAACCGTCCAGAAAG | Â |
RLF-ZMPSTE24 | RLF-exon1 | Forward | GTTGCCTACGCGCTGGTG | 2167Â bp |
| Â | ZMPSTE24-exon10 | Reverse | GATGTCCAGGATCTGTGACTGA | Â |